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| Joksic,G.; Petrovic,S.; Ilic,Z.. |
| The aim of the present study was to establish the extent of in vitro radioresponse of lymphocytes among 62 healthy adults of both genders and to estimate the distribution of baseline micronuclei and radiosensitivity among individuals of the study population using the cytochalasin block micronucleus test. A younger study group consisted of 10 males (mean age, 22.4 years; range, 21-27) and 12 females (mean age, 24.8 years; range, 20-29), whereas an older study group consisted of 18 males (mean age, 35.1 years; range, 30-44) and 22 females (mean age, 38.5 years; range, 30-48). For evaluation of radiosensitivity blood samples were irradiated in vitro using 60Co g-ray source. The radiation dose employed was 2 Gy, the dose rate 0.45 Gy/min. The study revealed a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human lymphocytes; Baseline micronuclei; Radiosensitivity; Ageing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800002 |
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| Antonucci,Gilmara Ausech; Takahashi,Catarina Satie. |
| The base analogue 2-chlorodeoxyadenosine (2-CdA) used for therapy in chronic resistant and advanced lymphoproliferative disorders, is cytotoxic for both dividing and non-dividing lymphocytes. The present work evaluated the clastogenic potential of this drug in vitro in human lymphocytes in culture and in vivo in BALB/c mice bone marrow cells. In human lymphocytes, the clastogenic effect of 2-CdA was studied in G1, S and G2 phases of the cell cycle, using three different concentrations (10, 20 and 40 mug/mL). The endpoints analyzed included mitotic index (MI), proliferation index (PI), sister chromatid exchange (SCE), and chromosomal aberration (CA). Statistical analysis by a variance (ANOVA) test showed a significant increase (p < 0.05) in CA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human lymphocytes; 2-CdA; Chromosomal aberrations; SCE; Cell cycle. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200027 |
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| PINCHEIRA,J; LÓPEZ,I; SANHUEZA,S; FERRUZ,P; NAVRRETE,MH; SANTOS,MJ; LÓPEZ-SÁEZ,JF. |
| The effect of the G2 repair of chromosomal damage in lymphocytes from workers exposed to low levels of X- or g-rays was evaluated. Samples of peripheral blood were collected from 15 radiation workers, 20 subjects working in radiodiagnostics, and 30 healthy control donors. Chromosomal aberrations (CA) were evaluated by scoring the presence of chromatid and isochromatid breaks, dicentric and ring chromosomes in lymphocytes with/without 5mM caffeine plus 3mM-aminobenzamide (3-AB) treatment during G2. Our results showed that the mean value of basal aberrations in lymphocytes from exposed workers was higher than in control cells (p< 0.001). The chromosomal damage in G2, detected with caffeine plus 3-AB treatment was higher than the basal damage... |
| Tipo: Journal article |
Palavras-chave: Chromosome aberrations ionizing radiation; G2 repair; Human lymphocytes; Occupational exposure. |
| Ano: 1999 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97601999000400009 |
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| Djelic,Ninoslav; Djelic,Dijana; Spremo-Potparevic,Biljana; Zivkovic,Lada; Markovic,Biljana; Lozance,Olivera; Blagojevic,Milos. |
| Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thyroxine; Human lymphocytes; Chromosome aberrations; Mitotic index; ROS. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019 |
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